Fluorometric determination of inulin using 5-quinolineboronic acid and inulinase.
نویسندگان
چکیده
Inulin is a polysaccharide composed mainly of D-fructose units and is the most reliable indicator of the glomerular filtration rate. We have proposed an inulin detection method that involves the hydrolysis of inulin to D-fructose using inulinase and the selective binding of D-fructose from inulin using 5-quinolineboronic acid. In this method, the fluorescence of 5-quinolineboronic acid increases, depending on inulin concentration. For inulin in plasma, the detection and quantitation limits were calculated to be 3.7 and 11 μg/ml, respectively.
منابع مشابه
Production of Inulinase by Fusarium sp. and its Application for Fructooligosaccharide Production for use as Prebiotics
Fructooligosaccharides (FOS) are useful due to their applications in food and pharmaceutical industry. Fusarium spp., isolated from dahlia rhizosphere, produced endoinulinases in a medium containing inulin or sucrose as carbon substrate. Characterisation of exo-inulinase and production of FOS were investigated. Temperature and pH optimum of the enzyme was found to be 60 °C and pH 6.0, respectiv...
متن کاملPurification, characterization, gene cloning and preliminary X-ray data of the exo-inulinase from Aspergillus awamori.
Extracellular exo-inulinase has been isolated from a solid-phase culture of the filamentous fungus Aspergillus awamori var. 2250. The apparent molecular mass of the monomer enzyme was 69 +/- kDa, with a pI of 4.4 and a pH optimum of 4.5. The enzyme hydrolysed the beta-(2-->1)-fructan (inulin) and beta-(2-->6)-fructan (levan) via exo-cleavage, releasing fructose. The values for the Michaelis con...
متن کاملProduction of inulinase from Kluyveromyces marxianus using dahlia tuber extract
Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia ext...
متن کاملThe pattern of action of inulinase from Saccharomyces fragilis on inulin.
Inulin is a naturally occurring plant polyfructoside. It consists of linear chains of approximately 35 n-fructose molecules, united by /?(2 + l)linkages, and terminated by a n-glucose molecule, which is linked to fructose by an OL(I --f 2)bond as in sucrose (1). In a previous paper (2) it was shown that Xaccharomyces fragilis excreted an inulin-hydrolyzing enzyme when the yeast was grown in a m...
متن کاملLocalization of inulinase and invertase in Kluyveromyces species.
In vivo hydrolysis of inulin and sucrose was examined in selected yeasts of the genus Kluyveromyces. Cells, grown in sucrose-limited chemostat cultures, were subjected to treatments for the removal of inulinase, the enzyme responsible for the hydrolysis of both inulin and sucrose. The effects of these treatments were studied by measurement of inulin-dependent and sucrose-dependent oxygen consum...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Analytical biochemistry
دوره 426 1 شماره
صفحات -
تاریخ انتشار 2012